Background: Medical schools in Brazil have been asked to adequate and reformulate their curriculums to include evidence-based medicine (EBM). The schools have contemplated this reality and answered the requirements solicited by the Guidelines of Brazilian National Curriculum for the Graduation Courses in Medicine. Objectives: To evaluate the prevalence of institutions which include EBM in their curricular teaching in Brazilian medical schools, as well as to identify their distribution across the regions that they are based in. Methods: Observational cross-sectional study (survey) of curriculums from Brazilian Medical Schools registered on the website of Anı´sio Teixeira National Institute of Educational Studies and Researches (Instituto Nacional de Estudos e Pesquisas Educacionais Anisio Teixeira-INEP), a public organ bound to the Education Ministry and responsible for following up the opening, accreditation and evaluation of graduate courses in Brazil. Data analysis were based on eight regional nucleus according to the Brazilian Association of Medical Education (Associac¸a˜o Brasileira de Educac¸a˜o Me´ dica/ABEM) as follows: North, Northeast, Central-East, Minas Gerais, South I, South II, Sa˜o Paulo, Rio de Janeiro, Rio de Janeiro/Espı´rito Santo. In the first phase of the study, all medical schools accredited in the INEP website were mapped, those which have included EBM in their electronic curriculums were identified. In the second phase, all the identified medical schools accredited by the INEP were sent a questionnaire by e-mail or fax. Six medical schools included the contents of EBM in their curriculums. Forty-nine out of 169 medical schools accredited in the INEP website answered the questionnaire of which only 23 were already including EBM in their curriculums. Conclusions: It was possible to conclude that all Brazilian regions have at least one medical school including EBM in its curriculum.