Background: The Knowledge to Action loop is a conceptual framework used to describe the movement of knowledge into action. It integrates the functions of knowledge creation, with that of knowledge application. This model was used as the basis for a 3-day advanced evidence-based practice course for practitioners in public health. Objectives: To increase the practitioners skills and knowledge in applying the cycle to public health practice, and to describe the challenges encountered. Methods: Three directly relevant case studies were developed before the workshop: an infectious disease response (communicable disease), addressing physical inactivity in a regional community (health promotion), and a community level intervention for food supply (public health nutrition). The knowledge to action cycle was taught in steps as lecturers, and applied by each disciplinary group. The groups undertook searches of the literature and applied critical appraisal tool whilst supported by their regional epidemiologists. Results: Participants struggled in defining the problem and identifying the applicable knowledge. The lack of systematic reviews specific to the topic increased the challenge of the task. For example, the public health nutritionists literature search only found a Cochrane registered title on food security. Each group was observed in accessing and assessing published literature throughout the workshop. Upon conclusion, each working group was able to present their response to the case-example using the framework. Conclusions: The challenges experienced were partly due to the complex nature of public health work, and also the lack of comprehensive synthesis research relevant to public health. The KT cycle appears to be a useful framework to public health although these is some scope for adaptation to the particular context. Knowledge translation is enhanced through the support of epidemiologists.