Article type
Abstract
Background: Group B streptococci was reported as a major cause of neonatal infection and death. Therefore the CDC guideline recommended that all women in week 35~37 of pregnancy should receive the Group B Streptococcus colonisation test to prevent vertical transmission to the newborn baby.
Objectives: We conducted a systematic review and meta-analysis to evaluate the diagnostic accuracy and detection rate of real-time PCR for Group B Streptococcus with pregnant women.
Methods:Searched KoreaMed, MEDLINE, EMBASE, and Cochrane Library on 3 November 2015.
Using strategic search with such keywords containing ‘Group B Streptococcus’, ‘real-time polymerase chain reaction’, a total of 441 articles were collected. Two authors independently select articles and evaluated the quality of studies using Scottish Intercollegiate Guidelines Network (SIGN).
Results: The diagnostic accuracy of the intervention test was assessed by meta-analysis through 34 articles (13,516 RT-PCR, 1,815 culture and other comparison test). In 4 articles where the quality assessment result was ‘++’, the intervention test was shown to have overall sensitivity of 0.93 (95% CI 0.91~0.95, p=.13, I2=44.4%), overall specificity of 0.96 (95% CI 0.95~0.97, p=.13, I2=43.8%), overall SROC of 0.98. In 30 articles where the quality assessment result was ‘+’, the diagnostic accuracy of the intervention test was shown to have overall sensitivity of 0.93 (95% CI 0.92~0.94, p=.00, I2=87.8%), overall specificity of 0.96 (95% CI 0.96~0.96, p=.00, I2=91.3%), overall SROC of 0.99.
The Group B Streptococcus colonisation was assessed through 34 articles, which reported varying values of 1.3~69.2%. One article reported the colonisation in pregnant Korean women to be 8.6% upon culture test and 12.6% upon real-time PCR by one article.
Conclusions: Group B Streptococcus Test [Real-Time PCR] is a effective test for detecting Group B Streptococcus colonisation in pregnant women using Real-Time PCR, thereby preventing the infection in a newborn baby (Grade of Recommendation C).
Objectives: We conducted a systematic review and meta-analysis to evaluate the diagnostic accuracy and detection rate of real-time PCR for Group B Streptococcus with pregnant women.
Methods:Searched KoreaMed, MEDLINE, EMBASE, and Cochrane Library on 3 November 2015.
Using strategic search with such keywords containing ‘Group B Streptococcus’, ‘real-time polymerase chain reaction’, a total of 441 articles were collected. Two authors independently select articles and evaluated the quality of studies using Scottish Intercollegiate Guidelines Network (SIGN).
Results: The diagnostic accuracy of the intervention test was assessed by meta-analysis through 34 articles (13,516 RT-PCR, 1,815 culture and other comparison test). In 4 articles where the quality assessment result was ‘++’, the intervention test was shown to have overall sensitivity of 0.93 (95% CI 0.91~0.95, p=.13, I2=44.4%), overall specificity of 0.96 (95% CI 0.95~0.97, p=.13, I2=43.8%), overall SROC of 0.98. In 30 articles where the quality assessment result was ‘+’, the diagnostic accuracy of the intervention test was shown to have overall sensitivity of 0.93 (95% CI 0.92~0.94, p=.00, I2=87.8%), overall specificity of 0.96 (95% CI 0.96~0.96, p=.00, I2=91.3%), overall SROC of 0.99.
The Group B Streptococcus colonisation was assessed through 34 articles, which reported varying values of 1.3~69.2%. One article reported the colonisation in pregnant Korean women to be 8.6% upon culture test and 12.6% upon real-time PCR by one article.
Conclusions: Group B Streptococcus Test [Real-Time PCR] is a effective test for detecting Group B Streptococcus colonisation in pregnant women using Real-Time PCR, thereby preventing the infection in a newborn baby (Grade of Recommendation C).