Background: The Cochrane Collaboration has recognized that evidence from qualitative studies can play an important role in adding value to systematic reviews for policy, practice and consumer decision-making. However, the role of qualitative evidence synthesis (QES) in training curricula of Cochrane centres/branches has not yet been evaluated. Methods: The Cochrane Qualitative Research Methods Group sent out a questionnaire to all Cochrane centres/ branches with the aim of: 1) mapping existing expertise within Cochrane centres/branches; 2) identifying activities that could stimulate an uptake of QES in Cochrane course programs; and 3) identifying existing training needs. Results: Ninety-five per cent of centres/branches responded to the questionnaire. Only 10% of the centres/branches offered QES courses and 20% employed staff members who had participated in QES course programs (75% at Cochrane Colloquia). Only one centre/branch had a staff member involved as a trainer, however, 20% claimed to have basic expertise in the field. The most urgent training needs involve critical appraisal of qualitative research and different synthesis approaches. Activities that would stimulate centres/branches to organise QES training include toolboxes, personal advice from co-convenors, individual guidance for authors and training in the Handbook material. The top training priorities were ‘train the trainer’ programs, downloadable course material and individual coaching within centres/branches. Conclusions: QES experience among Cochrane staff members is limited. Cochrane centres/branches might benefit from further training, guidance and resource development to stimulate uptake of QES in their course programs for authors of systematic reviews.