Background: Public health places strong emphasis on evidence-informed decision making (EIDM), which involves the incorporation of the best available research evidence along with other relevant sources of information for practice, program and policy decisions. Yet evidence indicates gaps between this ideal and actual practice. Objectives: To promote the consistent and routine use of the best available research evidence as well as local evaluation and surveillance data for tailored local decision making. Methods: Two program specialists (responsible for reviewing literature to address practice issues) from one health unit identified two priority practice questions and, supported by a knowledge broker, worked through the EIDM process to: formulate the questions; conduct comprehensive searches; access, appraise, and interpret the evidence; and discuss its adaptation for local implementation. Results: The practical, tailored approach to mentorship and skill development using issues of relevance to the individuals, their teams and organizations facilitated individual learning and team development; enabled the identification of organizational barriers to EIDM and intra-organizational knowledge sharing; and provided motivation for the development of policies, procedures, and related resources to address these barriers. Conclusions: This project, its process and outcomes, will be of interest to public health practitioners and managers, and others involved in knowledge translation and exchange. Participants will learn of an innovative approach to the promotion of EIDM at individual and organizational levels as well as strategies that may be transferable to their organization. Tools developed during this project will be made available to presentation attendees. Project results, related to individual and organizational development to support EIDM, will contribute to future knowledge translation and exchange activities at this health unit.